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red fluorescence intensity by clsm  (Olympus)


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    Olympus red fluorescence intensity by clsm
    Fig. 3 The effect of PS-MPs exposure on oxidative stress and inflammatory response in vivo. a Determination of ROS content by <t>CLSM</t> in control and PS- MPs exposure group. b Quantitative analysis of ROS described in a. c Representative IFA images of 4-HNE in the testis from three groups. d Comparative quantification of 4-HNE expression level mentioned in c. e IHA images showing the measurement of p65 in the testis treated with ddH2O and PS-MPs. f Quantification of p65-positive cells described in e. g-j Representative fluorescent images showing the detection of IL-1β and IL-16 and quantitative analysis of them. Scale bars: 50 μm in e; 30 μm in a, c, g and i
    Red Fluorescence Intensity By Clsm, supplied by Olympus, used in various techniques. Bioz Stars score: 99/100, based on 24505 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/red+fluorescence+intensity+by+clsm/FV3000+Confocal+Laser+Scanning+Microscope/pm37641072-120-12-17
    Average 99 stars, based on 24505 article reviews
    red fluorescence intensity by clsm - by Bioz Stars, 2026-10
    99/100 stars

    Images

    1) Product Images from "Long-term exposure to polystyrene microplastics triggers premature testicular aging."

    Article Title: Long-term exposure to polystyrene microplastics triggers premature testicular aging.

    Journal: Particle and fibre toxicology

    doi: 10.1186/s12989-023-00546-6

    Fig. 3 The effect of PS-MPs exposure on oxidative stress and inflammatory response in vivo. a Determination of ROS content by CLSM in control and PS- MPs exposure group. b Quantitative analysis of ROS described in a. c Representative IFA images of 4-HNE in the testis from three groups. d Comparative quantification of 4-HNE expression level mentioned in c. e IHA images showing the measurement of p65 in the testis treated with ddH2O and PS-MPs. f Quantification of p65-positive cells described in e. g-j Representative fluorescent images showing the detection of IL-1β and IL-16 and quantitative analysis of them. Scale bars: 50 μm in e; 30 μm in a, c, g and i
    Figure Legend Snippet: Fig. 3 The effect of PS-MPs exposure on oxidative stress and inflammatory response in vivo. a Determination of ROS content by CLSM in control and PS- MPs exposure group. b Quantitative analysis of ROS described in a. c Representative IFA images of 4-HNE in the testis from three groups. d Comparative quantification of 4-HNE expression level mentioned in c. e IHA images showing the measurement of p65 in the testis treated with ddH2O and PS-MPs. f Quantification of p65-positive cells described in e. g-j Representative fluorescent images showing the detection of IL-1β and IL-16 and quantitative analysis of them. Scale bars: 50 μm in e; 30 μm in a, c, g and i

    Techniques Used: In Vivo, Control, Expressing

    Fig. 4 Characterization and internalization of PS-MPs. a PS-MPs with 1 μm particle size observed under scanning electron microscope. b Fluorescent PS- MPs with 1 μm particle size observed under CLSM. c Internalization of PS-MPs in TM4 cells. Scale bars: 30 μm in b and 50 μm in c
    Figure Legend Snippet: Fig. 4 Characterization and internalization of PS-MPs. a PS-MPs with 1 μm particle size observed under scanning electron microscope. b Fluorescent PS- MPs with 1 μm particle size observed under CLSM. c Internalization of PS-MPs in TM4 cells. Scale bars: 30 μm in b and 50 μm in c

    Techniques Used: Microscopy

    Fig. 6 The effect of PS-MPs exposure on oxidative stress and Ca2+ signaling in TM4 cells. a Measurement of ROS levels in TM4 cells by CLSM. b Quantitative analysis of ROS presented in a. c, d Comparative statistics of MDA and SOD content between control group and PS-MPs exposure groups. e Represen tative fluorescent images showing Ca2+ signaling after PS-MPs exposure. f Quantification of fluorescent intensity of Ca2+ signaling as presented in e. g Representative fluorescent images of Ca2+ signaling and ROS levels in TM4 cells treated with control, PS-MPs and PS-MPs + BAPTA. h, i Comparative quan tification of fluorescent intensity of Ca2+ signaling (H) and ROS (I) as presented in g. The representative images were selected from three independent and replicated experiments (n = 3). Scale bars: 30 μm
    Figure Legend Snippet: Fig. 6 The effect of PS-MPs exposure on oxidative stress and Ca2+ signaling in TM4 cells. a Measurement of ROS levels in TM4 cells by CLSM. b Quantitative analysis of ROS presented in a. c, d Comparative statistics of MDA and SOD content between control group and PS-MPs exposure groups. e Represen tative fluorescent images showing Ca2+ signaling after PS-MPs exposure. f Quantification of fluorescent intensity of Ca2+ signaling as presented in e. g Representative fluorescent images of Ca2+ signaling and ROS levels in TM4 cells treated with control, PS-MPs and PS-MPs + BAPTA. h, i Comparative quan tification of fluorescent intensity of Ca2+ signaling (H) and ROS (I) as presented in g. The representative images were selected from three independent and replicated experiments (n = 3). Scale bars: 30 μm

    Techniques Used: Control

    Related Articles

    Fluorescence:

    Article Title: Long-term exposure to polystyrene microplastics triggers premature testicular aging.
    Article Snippet: .. The cells were then washed thrice with PBS before the detection of red fluorescence intensity by CLSM (Olympus FV3000) and quantified by ImageJ software. .. ATP Content Assay Kit (Solarbio, BC0300) was used for ATP content assay.

    Confocal Laser Scanning Microscopy:

    Article Title: Long-term exposure to polystyrene microplastics triggers premature testicular aging.
    Article Snippet: .. The cells were then washed thrice with PBS before the detection of red fluorescence intensity by CLSM (Olympus FV3000) and quantified by ImageJ software. .. ATP Content Assay Kit (Solarbio, BC0300) was used for ATP content assay.



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    red fluorescence intensity by clsm  (Olympus)
    99
    Olympus red fluorescence intensity by clsm
    Fig. 3 The effect of PS-MPs exposure on oxidative stress and inflammatory response in vivo. a Determination of ROS content by <t>CLSM</t> in control and PS- MPs exposure group. b Quantitative analysis of ROS described in a. c Representative IFA images of 4-HNE in the testis from three groups. d Comparative quantification of 4-HNE expression level mentioned in c. e IHA images showing the measurement of p65 in the testis treated with ddH2O and PS-MPs. f Quantification of p65-positive cells described in e. g-j Representative fluorescent images showing the detection of IL-1β and IL-16 and quantitative analysis of them. Scale bars: 50 μm in e; 30 μm in a, c, g and i
    Red Fluorescence Intensity By Clsm, supplied by Olympus, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/red+fluorescence+intensity+by+clsm/FV3000+Confocal+Laser+Scanning+Microscope/pm37641072-120-12-17
    Average 99 stars, based on 1 article reviews
    red fluorescence intensity by clsm - by Bioz Stars, 2026-10
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    Fig. 3 The effect of PS-MPs exposure on oxidative stress and inflammatory response in vivo. a Determination of ROS content by CLSM in control and PS- MPs exposure group. b Quantitative analysis of ROS described in a. c Representative IFA images of 4-HNE in the testis from three groups. d Comparative quantification of 4-HNE expression level mentioned in c. e IHA images showing the measurement of p65 in the testis treated with ddH2O and PS-MPs. f Quantification of p65-positive cells described in e. g-j Representative fluorescent images showing the detection of IL-1β and IL-16 and quantitative analysis of them. Scale bars: 50 μm in e; 30 μm in a, c, g and i

    Journal: Particle and fibre toxicology

    Article Title: Long-term exposure to polystyrene microplastics triggers premature testicular aging.

    doi: 10.1186/s12989-023-00546-6

    Figure Lengend Snippet: Fig. 3 The effect of PS-MPs exposure on oxidative stress and inflammatory response in vivo. a Determination of ROS content by CLSM in control and PS- MPs exposure group. b Quantitative analysis of ROS described in a. c Representative IFA images of 4-HNE in the testis from three groups. d Comparative quantification of 4-HNE expression level mentioned in c. e IHA images showing the measurement of p65 in the testis treated with ddH2O and PS-MPs. f Quantification of p65-positive cells described in e. g-j Representative fluorescent images showing the detection of IL-1β and IL-16 and quantitative analysis of them. Scale bars: 50 μm in e; 30 μm in a, c, g and i

    Article Snippet: The cells were then washed thrice with PBS before the detection of red fluorescence intensity by CLSM (Olympus FV3000) and quantified by ImageJ software.

    Techniques: In Vivo, Control, Expressing

    Fig. 4 Characterization and internalization of PS-MPs. a PS-MPs with 1 μm particle size observed under scanning electron microscope. b Fluorescent PS- MPs with 1 μm particle size observed under CLSM. c Internalization of PS-MPs in TM4 cells. Scale bars: 30 μm in b and 50 μm in c

    Journal: Particle and fibre toxicology

    Article Title: Long-term exposure to polystyrene microplastics triggers premature testicular aging.

    doi: 10.1186/s12989-023-00546-6

    Figure Lengend Snippet: Fig. 4 Characterization and internalization of PS-MPs. a PS-MPs with 1 μm particle size observed under scanning electron microscope. b Fluorescent PS- MPs with 1 μm particle size observed under CLSM. c Internalization of PS-MPs in TM4 cells. Scale bars: 30 μm in b and 50 μm in c

    Article Snippet: The cells were then washed thrice with PBS before the detection of red fluorescence intensity by CLSM (Olympus FV3000) and quantified by ImageJ software.

    Techniques: Microscopy

    Fig. 6 The effect of PS-MPs exposure on oxidative stress and Ca2+ signaling in TM4 cells. a Measurement of ROS levels in TM4 cells by CLSM. b Quantitative analysis of ROS presented in a. c, d Comparative statistics of MDA and SOD content between control group and PS-MPs exposure groups. e Represen tative fluorescent images showing Ca2+ signaling after PS-MPs exposure. f Quantification of fluorescent intensity of Ca2+ signaling as presented in e. g Representative fluorescent images of Ca2+ signaling and ROS levels in TM4 cells treated with control, PS-MPs and PS-MPs + BAPTA. h, i Comparative quan tification of fluorescent intensity of Ca2+ signaling (H) and ROS (I) as presented in g. The representative images were selected from three independent and replicated experiments (n = 3). Scale bars: 30 μm

    Journal: Particle and fibre toxicology

    Article Title: Long-term exposure to polystyrene microplastics triggers premature testicular aging.

    doi: 10.1186/s12989-023-00546-6

    Figure Lengend Snippet: Fig. 6 The effect of PS-MPs exposure on oxidative stress and Ca2+ signaling in TM4 cells. a Measurement of ROS levels in TM4 cells by CLSM. b Quantitative analysis of ROS presented in a. c, d Comparative statistics of MDA and SOD content between control group and PS-MPs exposure groups. e Represen tative fluorescent images showing Ca2+ signaling after PS-MPs exposure. f Quantification of fluorescent intensity of Ca2+ signaling as presented in e. g Representative fluorescent images of Ca2+ signaling and ROS levels in TM4 cells treated with control, PS-MPs and PS-MPs + BAPTA. h, i Comparative quan tification of fluorescent intensity of Ca2+ signaling (H) and ROS (I) as presented in g. The representative images were selected from three independent and replicated experiments (n = 3). Scale bars: 30 μm

    Article Snippet: The cells were then washed thrice with PBS before the detection of red fluorescence intensity by CLSM (Olympus FV3000) and quantified by ImageJ software.

    Techniques: Control